Predictive Factors for Successful Testicular Biopsy in Azoospermia

Authors

  • Awatef Raddaoui Department of Obstetrics and Gynaecology, Tunis Main Military Teaching Hospital, Faculty of Medicine of Tunis, University of Tunis El Manar, 1007, Tunis, Tunisia
  • Omar Abdelhedi Department of Obstetrics and Gynaecology, Tunis Main Military Teaching Hospital, Faculty of Medicine, University of Sfax, Sfax, Tunisia
  • Dorra Boujelben Department of Obstetrics and Gynaecology, Tunis Main Military Teaching Hospital, Faculty of Medicine of Tunis, University of Tunis El Manar, 1007, Tunis, Tunisia
  • Sonia Jalled Department of Obstetrics and Gynaecology, Tunis Main Military Teaching Hospital, Faculty of Medicine of Tunis, University of Tunis El Manar, 1007, Tunis, Tunisia

DOI:

https://doi.org/10.62438/tunismed.v104i02.6530

Keywords:

Testicular biopsy, Azoospermia, Predictive factors, FSH, Spermogram

Abstract

Introduction: Testicular biopsy represents a crucial procedure in the management of non-obstructive azoospermia, though its success remains
variable. Identifying predictive factors for successful sperm retrieval is essential for patient selection.
Aim: To determine the clinical, hormonal and technical factors predictive of successful testicular biopsy in azoospermic patients
Methods: A retrospective analytical study was conducted over 5 years (2017-2021), including 28 patients. Comparative analysis was performed
between groups with successful (spermatozoa present) and failed biopsy.
Results: Significant predictive factors for successful biopsy included: testicular volume preservation (p=0.042), low FSH levels (p=0.002), low
estradiol levels (p=0.018), excretory azoospermia type (p=0.023), and unilateral surgical approach (p=0.005). Clinical varicocele and hydrocele
showed no significant correlation.
Conclusion: Our study identifies a predictive profile for testicular biopsy success, enabling better patient selection and surgical strategy optimization
in assisted reproductive technology.

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References

Jarow JP, Espeland MA, Lipshultz LI. Evaluation of the azoospermic patient. J Urol. 1989;142(1):62-5.

Leke RJ, Oduma JA, Bassol-Mayagoitia S, et al. Regional and geographical variations in infertility: effects of environmental, cultural, and socioeconomic factors. Environ Health Perspect. 2021;129(6):067001.

Schlegel PN. Diagnosis and treatment of obstructive azoospermia. Reprod Med Rev. 1995;4(2):97-108.

Charny CW. Testicular biopsy. Its value in male sterility. JAMA. 1940;115(17):1429-32.

Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340(8810):17-8.

Practice Committee of the American Society for Reproductive Medicine. Management of nonobstructive azoospermia: a committee opinion. Fertil Steril. 2018;110(7):1239-45.

Bernie AM, Mata DA, Ramasamy R, Schlegel PN. Comparison of microdissection testicular sperm extraction, conventional testicular sperm extraction, and testicular sperm aspiration for nonobstructive azoospermia: a systematic review and meta- analysis. Fertil Steril. 2015;104(5):1099-103.

Ouedraogo C, Thiam A, Diop A, et al. Testicular sperm extraction outcomes in West African patients with non-obstructive azoospermia. Afr J Urol. 2021;27(1):45.

Adebayo AA, Oyeyipo IP, Du Plessis SS. Male infertility in Africa: a systematic review and meta-analysis of testicular sperm extraction outcomes. Pan Afr Med J. 2022;41:187.

Deruyver Y, Vanderschueren D, Van der Aa F. Outcome of microdissection TESE compared with conventional TESE in

non-obstructive azoospermia: a systematic review. Andrology. 2014;2(1):20-4.

Ramasamy R, Lin K, Gosden LV, et al. High serum FSH levels in men with nonobstructive azoospermia does not affect success of microdissection testicular sperm extraction. Fertil Steril. 2009;92(2):590-3.

Bujan L, Mansat A, Pontonnier F, Mieusset R. Time series analysis of sperm concentrations in fertile men in Toulouse, France between 1977 and 1992. BMJ. 1996;312(7029):471-2.

Bakare AT, Adebayo AA, Olaomo NO. Pattern of male infertility in South-West Nigeria: the need for public health intervention. Afr J Reprod Health. 2020;24(3):145-152.

Verheyen G, Popovic-Todorovic B, Vernaeve V. Processing and selection of surgically retrieved sperm for ICSI: a review. Basic Clin Androl. 2017;27:6.

Mbarki C, Sellami A, Ouzazen F, et al. Factors predicting successful sperm retrieval in patients with non-obstructive azoospermia in Tunisia. Tunis Med. 2021;99(2):258-263.

Oates RD, Mulhall J, Burgess C, et al. Fertilization and pregnancy using intentionally cryopreserved testicular tissue as the sperm source for intracytoplasmic sperm injection in 10 men with non- obstructive azoospermia. Hum Reprod. 1997;12(4):734-9.

Faye M, Diop A, Kouame A, et al. Development of a predictive score for successful sperm retrieval in West African men with non- obstructive azoospermia. Andrology. 2023;15(2):345-352.

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Published

2026-04-01

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