Etablissement des valeurs de référence des lymphocytes T chez les donneurs de sang parcytométrie en flux
Mots-clés :
CD3+/CD4+; CD3+/CD8+; cytométrie de flux; sous populations lymphocytaires T; valeurs de référence.Résumé
Objectifs : Déterminer les valeurs de référence des sous-populations lymphocytaires T par cytométrie de flux dans une population de donneurs de sang de la région de Sfax et évaluer l’influence de l’âge et du sexe sur leurs numérations.
Méthodes : L’étude a porté sur 143 donneurs de sang du centre régional de transfusion sanguine de Sfax. Les valeurs de référence ont été déterminées par technique double plate-forme (compteur de globules pour la numération en valeur absolue des lymphocytes circulants et cytomètre EPICS XL pour l'évaluation en pourcentages des lymphocytes T CD3+, CD3+/CD4+ et CD3+/CD8+). L'analyse statistique a été faite par les tests de Student et d’ANOVA.
Résultats : Les valeurs de référence des sous populations lymphocytaires T ont été exprimées en moyenne et en intervalles de confiance à 95%: CD3+ : 1415 ± 348 cellules/μL [1357–1473], CD3+/CD4+ : 786 ± 220 cellules/μL [732,31–811,7], CD3+/CD8+ : 639 ± 258 cellules/μL [596-862] avec un ratio CD4+/CD8+ de 1,46 ± 0,77 [1,36–1,62]. Elles sont influencées par l'âge et le sexe.
Conclusion : Notre étude a établi les valeurs de référence des lymphocytes T chez les donneurs de sang dans la région de Sfax. D’autres études plus large et portant sur d'autres régions de la Tunisie et sur les autres populations lymphocytaires B et NK sont fortement recommandées.
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Références
Helbert M, Breuer J. Monitoring patients with HIV disease. J Clin Pathol 2000; 53 (4): 266-72.
Masur H, Ognibene FP, Yarchoan R, Helhamer JH, Baird BF, Travis W and al. CD4 counts as predictors of opportunistic pneumonias in human immunodeficiency virus (HIV) infection. Ann Intern Med 1989; 111 (3):223-31.
Brando B, Barnett D, Janossy G, Mandy F, Autran B, Rothe G and al. Cytofluorometric methods for assessing absolute numbers of cell subsets in blood. European Working Group on clinical cell analysis. Cytometry 2000; 42 (6) :327-46.
Anaes. Service des références médicales. Lecture critique de l'hémogramme : valeurs seuils à reconnaître comme probablement pathologiques et principales variations non pathologiques. 1997: 309-44.
Lee BW, Yap HK, Chew FT, Quah TC, Prabhakaran K, Chan GS and al. Age- and sex-related changes in lymphocyte subpopulations of healthy Asian subjects: from birth to adulthood. Cytometry 1996; 26: 8-15.
Chng, WJ, Tan GB, and Kuperan P. Establishment of adult peripheral blood lymphocyte subset reference range for an Asian population by single-platform flow cytometry: influence of age, sex, and race and comparison with other published studies. Clin. Diagn. Lab Immunol 2004; 11: 168-73.
Ben Amor I, Menif H, Hamida A, Gdoura I, Rekik H, Gargouri J. Hemogram reference values: study of 1000 healthy adults from Sfax. Arch Inst Pasteur Tunis 2012; 89 (1-4): 47.
Uppal SS, Verma S, Dhot PS: Normal values of CD4 and CD8 lymphocyte subsets in healthy Indian adults and the effects of sex, age, ethnicity,and smoking. Cytometry B Clin Cytom. 2003; 52B:32-36.
Santagostino A, Garbaccio G, Pistorio A, Bolis V, Camisasca G, Pagliaro P and al. An Italian national multicenter study for the definition of reference ranges for normal values of peripheral blood lymphocyte subsets in healthy adults. Haematologica 1999; 84: 499-504.
Guidelines for Performing Single-Platform Absolute CD4+ T-Cell Determinations with CD45 Gating for Persons infected with human immunodeficiency virus. MMWR CDC 2003; 52(RR02):1-13.
Singh YG, Dar L, Singh NG. Levels of CD4 and CD8 among the inhabitants of Manipur, India. The Journal of communicable diseases 2000; 32(3):201-06.
Yamana A, Çetinerb S, Kibara F, Tasova Y, Seydaglu G, Dündar I. Reference Ranges of Lymphocyte Subsets of Healthy Adults in Turkey. Med Princ Pract 2005; 14:189-193.
Lugada E S, Mermin J , Kaharuza F, Ulvestad E, Were W, Langeland N and al . Population-based hematologic and immunologic reference values for a healthy Ugandan population. Clin Diagn Lab Immunol 2004; 11(1), 29-34.
Ronot X, Grunwald D,Mayol JF,Boutonnat J. La Cytométrie en flux. Paris,France: Lavoisier 2006: 99-151.
Jentsch-Ullrich K, Koenigsmann M, Mohren M, Franke A. Lymphocyte subsets reference ranges in an age-and gender-balanced population of 100 healthy adults, a monocentric German study. Clin Immunol 2005; 116(2):192-97.
Bisset LR, Lung TL, Kaelin M, Ludwig E, Dubs RW. Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland. Eur j haematol 2004; 72(3): 203-12.
Comans-Bitter WM, De Groot R, Van den Beemed R, Neijens HJ, Groeneveld H and al, Immunophenotyping of blood lymphocytes in childhood. J Pediatr1997; 130.
Tsegaye A, Messele T, Tilahun T, Hailu E, Sahlu T, Doorly R and al. Immunohematological reference ranges for adult Ethiopians. Clin Diagn Lab Immunol 1999; 6: 410 - 414.
Al Qouzi A, Al Salamh A, Al Rasheed R, Al Muslam A, Al Kairy K, Kheir O and al, Immunophenotyping of peripheral blood lymphocyte in Saudi arabi men, Clin Diagn. Lab immunol. 9,279.
Valiathan R, Dheeb K, Diamante M Ashman M, Sachdeva N, Asthana D. Reference ranges of lymphocyte subsets in healthy adults and adolescents with special mention of T cell maturation subsets in adults of south florida. Immunobiology 2013; 487-496.
Al-Mawali A, Daniel Pinto A, Al Busaidi R, Al-Zakwani I. Lymphocyte Subsets: Reference ranges in an age- and gender-balanced population of Omani Healthy Adults. Cytometry Part A 2013; 83A: 739 -744
Alamooti A, Ardalan FA, Abdolahi A, Zeidi M, Firouzjaie F. Determination of lymphocyte subsets reference values in healthy Iranian men by a single platform flow cytometric method. Cytometry Part A 2010; 77(9): 890-94.
Bouman A, Heineman MJ, Faas MM. Sex hormones and the immune response in humans. Hum. Reprod. Update 2005; 11:411-23.
Oertelt Prigione S. The influence of sex and gender on the immune response. Autoimmun Rev 2012; 11 (6): A479-A485.
Chng WJ, Tan GB, Kuperan P. Establishment of adult peripheral blood lymphocyte subset reference range for an Asian population by single-platform flow cytometry: influence of age, sex, and race and comparison with other published studies. Clin diagn lab immune 2004; 11(1), 168-73.
Tollerud DJ, Ildstad ST, Brown LM, Clark JW, Blattner WA, Mann DL and al. T-Cell subsets in healthy teenagers: transition to the adult phenotype .Clin ,Immunol.Immunopathol 1990;56 (1):88 - 96.
Linton PJ, Dorshkind K. Age-related changes in lymphocyte development and function. Nat immunol. 2004; 5:133-39.
Denny T, Yogev R, Gelman R, and al. Lymphocyte subsets in healthy children during the first 5 years of life. Jama 1992; 267(11): 1484-88.
Erkeller-Yuksel FM, Deneys V, Yuksel B, Hannet I, Hulstaert F, Hamilton C and al. Age-related changes in human blood lymphocyte subpopulations. J Pediatr 1992; 120:216-22.
Shearer WT, Rosenblatt HM, Gelman RS, Oyomopito R, Plaeger S, Stiehm ER and al. Lymphocyte subsets in healthy children from birth through 18 years of age: The Pediatric AIDS Clinical Trials Group P1009 Study, J Allergy Clin Immunol 2003; 112 (5)973 - 980.
